The Production of Free Staphylococcal Coagulase

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Purification of staphylococcal coagulase.

Correlation between the pathogenicity of certain staphylococci and their ability to produce coagulase has led to extensive studies of the clotting enzyme. Walston (1935) precipitated the clotting substance from cell-free filtrates of appropriate staphylococcal cultures by the use of ethanol, acetic acid, or ammonium sulfate. Walker et al. (1948) partially purified staphylocoagulase by repeated ...

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Staphylococcal coagulase; mode of action and antigenicity.

Different strains of staphylococci vary in ability to produce free coagulase. Methods are given for the selection of suitable strains and for the production and concentration of coagulase from one of them. Of animal plasmas examined, cow, sheep, dog, guinea-pig and mouse showed a relative deficiency in coagulase activator, while plasma of man, monkey, horse, cat, pig, fowl and rabbit contained ...

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Factors affecting the demonstration of staphylococcal free coagulase and clumping factor.

With the use of Phenol Red Mannitol (PRM) Broth (Difco) as a basal medium, rather than Trypticase Soy (TS) Broth (BBL), we were able to modify a previously described plasma-fibrinogen-soft agar medium (S. Y. Alami and F. C. Kelly, J. Bacteriol. 78:539, 1959) for the simultaneous demonstration of mannitol fermentation (MF), free coagulase (FC), and clumping factor (CF) production by staphylococc...

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‏‎facilitating lexical access for the fluent production of speech‎‏

‏‎the hypothesis is that recent and frequent exposure to lexical items leads to a more fluent production of speech in terms of rate of speech. to test the hypothesis , a one- way anova experimental design was carried out. 24 senior student of efl participated in a one-way interview test. data analyses revealed that those who were exposed frequently to the lexical items over a week prior to inte...

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ژورنال

عنوان ژورنال: Journal of General Microbiology

سال: 1954

ISSN: 0022-1287

DOI: 10.1099/00221287-10-3-437